Characterisation of Arabidopsis thaliana candidate genes putatively involved in the response to salt stress

Kasemsap, P. 2015. Characterisation of Arabidopsis thaliana candidate genes putatively involved in the response to salt stress. Master thesis. (MSc. in Plant Sciences at Wageningen University, the Netherlands).

Abstract

Salinity is one of the most severe abiotic constraints in plant production worldwide. Prior to this master’s thesis, candidate genes presumed to underlie salt stress responses in Arabidopsis thaliana were identified in the GWAS of 350 accessions collected from diverse ecosystems. Yet, these genes need to be validated for their putative functions. To this end, Arabidopsis thaliana T-DNA insertion mutants of selected candidate genes were phenotyped in salt stress experiments conducted in both on rockwool and on agar plates. Plants on rockwool were sub-irrigated with 0, 25 and 75 mM NaCl solution for 4 weeks. Plants on agar plates were grown on solid media containing 0, 25, 75 and 125 mM NaCl for 17 days. Mutants of AT1G77600, AT3G49600 and AT3G49601 demonstrated altered salt stress responses in comparison to wild type in both assays. This suggests these candidate genes might involve in salt tolerance mechanisms. In addition, promoter (500 bp) and artificial RNA constructs of AT1G77600 were successfully cloned to be transformed into Arabidopsis thaliana for further functional analysis. Further validation is still required to confirm the gene functions before concluding that these genes indeed underlie salt stress responses in Arabidopsis thaliana.

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tag: science  plant  gradschool  publication 

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